RT-MLPSEQ: A new technology to characterize cancers Gene expression and fusion transcripts
The RT-MLPSeq custom solution from CliniSciences and GeneXpath allows you to assess the mRNA expression level or the presence of fusion transcripts of 10 to 100 markers of interest of your choice.
RT-MLPSeq Custom Solution
In research projects, preclinical and clinical studies, it is often necessary to obtain the gene expression or the presence of fusion transcripts of several dozen markers. At present, relative expression and fusion transcripts can only be analyzed with a small number of genes simultaneously, using complicated protocols. In addition, manipulation times are often very long, and solutions on the market are often expensive.
Genexpath's Custom Panel
The Genexpath custom panel solution enables you to select your markers of interest quickly and easily. We then provide you with a turnkey solution: an in Vitro test and a bioinformatic analysis after NGS sequencing.
These custom panels can be produced for any type of genome. We are at your disposal to produce panels of between ten and a hundred markers.
RT-MLPSEQ Advantages:
- Simple and fast protocol: ½ day manipulations.
- Small quantity of material required: Only 50–500 ng of RNA needed. Compatible with fresh, frozen, or FFPE samples (tissue, liquid, etc.). Optimized for difficult samples like FFPE.
- No purification required: Limits material losses and ensures excellent sensitivity.
- Robustness of the technique.
Applications in Oncology Diagnostics
RT-MLPSeq technology is already being used in oncology diagnostic kits for:
- Characterisation of non-Hodgkin's lymphomas: According to the WHO classification, by precise measurement of the expression signature of more than 130 genes with our LymphoSign test (CE-IVD).
- Characterisation of peripheral T-cell lymphomas: By analyzing fusion transcripts using our LymphoTranscript test.
- Characterisation of sarcomas: By analyzing over 140 fusion transcripts using our SarcomaFusion test (CE-IVD).
RT-MLPSEQ: A Simple and Rapid Protocol
- The in vitro test consists of 4 steps lasting ½ day, including 1–1.5 hours of handling time.
- NGS sequencing requires only 100,000 reads per sample.
- Samples can be sequenced alongside other libraries, and barcodes are included in the kit.
- These tests save significant time in your projects.
Robust and Efficient Computer Analysis
Once sequencing is completed, you can upload the FASTQ file onto the Genexpath platform. Within minutes, you receive a file containing raw data and the relative expression of each marker.
On request, RT-MIS can also generate customized graphs to facilitate result interpretation.