Reagenzien und Instrumente für die Immunologie Zellbiologie und Molekularbiologie
 

Custom Peptide Synthesis

Peptides are used for a wide range of applications, from polyclonal antibody production to development of therapeutic molecules
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Custom peptide production service offers the synthesis and purification of peptides. This peptide synthesis can be accompanied by modifications at the N or C terminal positions.

Peptide synthesis typically involves coupling the carboxyl group of an incoming amino acid to the N-terminus of the growing peptide chain, following a C-to-N direction. This approach contrasts with protein biosynthesis, where amino acids are added in the N-to-C direction by linking the N-terminus of the incoming amino acid to the C-terminus of the growing protein chain. In vitro peptide synthesis, due to its complexity, proceeds in a precise, stepwise, and cyclic manner. Despite variations among commonly used peptide synthesis methods, they all share this sequential approach, adding amino acids one at a time to the peptide chain.


The peptide synthesis service adapts to your request:

  • Simple linear peptides
  • Complex peptides; cyclic,
  • Long chain peptides (up to 112 amino acids)
  • Modified amino acid containing peptides
  • Various grades of purity: crude, desalted, >75%、>85%、>90%、>95%、>98%.

 

 

Choose the purity of your peptide according to your needs:

Crude (>50 >70%)

 

  • High-throughput screening of lead compounds.
  • Mutation screening
  • Sequence optimization

 

Immuno-grade purity (>75%)

 

  • ELISA testing
  • Peptide arrays
  • Production, purification and testing of antibodies used in immunoassays

 

Biochemistry grade ( >85%)

 

  • For in-vitro bioassays and semi-quantitative applications (non-quantitative) such as studies in enzymology, epitope mapping, biological activity, enzyme-substrate interactions, or phosphorylation, as well as for peptide blocking studies for Western Blot and cell attachment.
  • Antigens for polyclonal antibody production
  • Coupling to chromatography resins for affinity purifications

 

High purity: >90% to >98%

 

  • Production of monoclonal antibodies
  • In vitro quantitative studies such as receptor-ligand interaction studies, enzyme-substrate and other biological activity studies, phosphorylation and proteolysis studies
  • Quantitative blocking and competitive inhibition assays, electrophoresis markers and chromatography standards
  • Quantitative bioassays (ELISA and RIA)
  • In vivo studies

 

Extremely high purity: >98%

 

  • In vivo studies, clinical trials, drug studies that use peptides as pharmaceuticals and cosmetic peptides for cosmeceuticals industry
  • Structure-activity relationship studies (enzyme-substrate studies, receptor-ligand interaction studies…)
  • Protein structure studies by nuclear magnetic resonance or protein crystallography.

 



The average time to synthesize a peptide is 2 to 3 weeks.

The peptide is analyzed by mass spectrometry, HPLC and is delivered with a synthesis report.
 

Peptides may have the following modifications (non-exhaustive list) :

 

  • In N-terminal
  • In C terminal
  • Non-usual amino acids
  • Conjugation